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KMID : 0861020180330020019
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Korea Journal of Herbology
2018 Volume.33 No. 2 p.19 ~ p.28
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nti-inflammatory Effects of Epimedii Herba Water Extract through Inhibition of Nuclear Factor-κB in RAW 264.7 Cells
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Jung Ji-Yun
Byun Sung-Hui
Park Chung-A
Cho Il-Je
Kim Sang-Chan
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Abstract
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Objectives : Epimedii Herba has been frequently used in Korean Traditional Medicine to treat impotence, spermatorrhoea, exophthalmos, and forgetfulness. Present study investigated anti-inflammatory effects of Epimedii Herba water extract (EWE) and attempted to elucidate molecular mechanisms involved.
Methods : To explore anti-inflammatory effects of EWE, RAW 264.7 cells, a murine macrophage cell line, were pretreated with 10-100 ㎍/㎖ of EWE, and then subsequently exposed to 1 ㎍/㎖ of lipopolysaccharide (LPS). Levels of nitric oxide (NO), interleukin-6, interleukin-1β, and tumor necrosis factor-α were monitored in the medium. Expression levels of inducible nitric oxide synthase and cyclooxygenase-2 were determined by immunoblot and real-time PCR analyses. Signaling pathways related with nuclear factor-κB (NF-κB) and mitogen-activated protein kinases were monitored to elucidate molecular mechanisms involved. Finally, the role of three flavonoid compounds in EWE on LPS-mediated NO production were investigated.
Results : In conditioned medium, pretreatment of EWE (100 ㎍/㎖) significantly inhibited LPS-stimulated NO and pro-inflammatory cytokine production. In addition, EWE attenuated the expressions of inducible nitric oxide synthase and cyclooxygenase-2 by LPS. EWE prevented the phosphorylation and degradation of inhibitory κBα, nuclear translocation of NF-κB, and DNA binding of NF-κB, while EWE did not change the phosphorylation of mitogenactivated protein kinases by LPS. Moreover, icariin, icaritin, and quercetin partly, but significantly, inhibited the LPS-stimulated NO production.
Conclusions : These results suggest that EWE has an ability to prevent inflammation in macrophages through inhibition of NF-κB signaling pathway.
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KEYWORD
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Anti-inflammation, Epimedii Herba, Lipopolysaccharide (LPS), Nuclear factor-κB (NF-κB), RAW 264.7 cell
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